Project Partner 5: CNRS-INEE: Institute of Ecology and Environment

Understanding the biosphere: the key to action

At the interface of social, earth and life sciences, the CNRS Institute of Ecology and Environment (INEE) has developed an integrative approach to environmental sciences that aims to promote global ecology at every scale of time and space. INEE is a fundamental research institute that combines research with action, and contributes to the advancement of knowledge for sustainable development.

Mission and goals

INEE's mission is to bring the environmental sciences to the fore as an integrated scientific field. Its goals are to promote creativity and undertake fundamental research of excellence, and to set up and develop innovative tools in global ecology through the development of partnerships. Research is carried out by a network of laboratories in the fields of evolutionary science, biodiversity, ecology and human-environment interactions.

LEMAR (Laboratoire des sciences de l’environnement marin) Brest – UMR 6539

The scientific objective of LEMAR focuses on understanding the mechanisms that regulate the functioning of marine ecosystems and their response to the multiple facets of global change. The laboratory is structured into three teams, Team 1, "Responses of organisms to global change: an integrative approach" Team 2 " biotic interactions and environmental variability," Team 3 "Integrated study of ecosystem functioning." Researchers associated with the project MICRO mainly belong to the team 2 that aims to better understand trophic and species interactions, taking into account environmental variability. Researchers involved in MICRO are particularly specialized in cellular responses upon environmental variability (biotic and abiotic). They have accumulated more than ten years experience on developing tools to evaluate morpho-functional parameters of bivalve hemocytes by flow cytometry.

For MICRO, the LEMAR has the equipments and skills necessary for patho-physiological analysis by histology and functional analysis of hemocytes by flow cytometry, analysis of molecular biology by quantitative RT-PCR, analysis of lipids by HPTLC, analysis of enzymes by spectrophotometry, a molecular biology laboratory for all DNA and RNA extraction and preparation. Finally, for the realization of experiments, the LEMAR has structures of different sizes, control of the culture quality and the ability to implement complex experimental systems.

Project team

Philippe Soudant is bivalve physiologist. Involved in all activities

Philippe SoudantPhilippe Soudant is director of research at CNRS-INEE, HDR since 2005. He received his Ph D in marine biology from the University of Brest in 1995. He has over 17 years experience in the fields of ecology and physiology of bivalves. For MICRO, he is the scientific responsible of the partner CNRS.
He has been already the scientific coordinator in several national, European and international projects. He is (co)author of 2 book chapters and 90 peer-reviewed articles. He is member of the editorial boards of Journal of Shellfish Research.

Marc Long is an engineer assistant in marine biology. Involved in activity 2.

Marc LongLong is an engineer assistant working for the CNRS-INEE at the “Institut Universitaire Européen de la Mer”. He began to work on physiology of toxic phytoplankton. He tries now to develop methods to see the effects of microplastics on oysters and microalgae by flow cytometry and microscopy. He is also working on the possibility to use phytoplankton as a vector of microplastics for bivalves.

Nelly Le Goïc is an engineer assistant in marine biology at CNRS. Involved in activity 2.

Nelly Le GoïcNelly Le Goïc is working at CNRS since 2004. In the framework of the MICRO project, she performs analyses at cellular level on bivalves using flow cytometry, microscopy and histology. She also develops protocols to extract and quantify microplastics in biota.

Christophe Lambert is a research engineer at CNRS. Involved in activity 2.

Christophe LambertChristophe Lambert is in charge of the cytometry tools at LEMAR : mainly three flow-cytometers and two inverted microscopes with fluorescence illumination systeme of which one is equipped with a 3D acquisition system. He hold a PhD in Biological Oceanology from the Western Brittany University on bivalve diseases (vibriosis in Pecten maximus larvae) since 1998 and his HDR (accreditation to supervise research) since 2007. He has over 15 years experience in the fields of physiology, immunology and bacterial diseases of bivalves in relation to environmental changes. He is (co)author of 45 peer-reviewed articles. For MICRO, he is responsible of flow-cytometric and microscopic analyses about the mechanisms involved in MP impacts on oysters, especially on hemocyte activities, and on micro-algae.

Hélène Hégaret is bivalve physiologist. Involved in activity 2.

Hélène HégaretHélène Hégaret is research scientist at CNRS-INEE, since 2009. She received her Ph D in oceanography and fishery sciences from the University of Connecticut, USA, and the Ecole Nationale Supérieure Agronomique de Rennes, France. She has over 10 years experience with the field of bivalve physiology, immunology and cellular biology. She has also more recently developed expertise in phytoplankton biology and physiology. She is (co)author of 1 book chapter and 27 peer-reviewed articles. For MICRO, she is involved in activity 2. She has already been the scientific coordinator on national and international research projects.

Anne-Laure Cassone is an assistant engineer in marine biology. Involved in activity 2.

Anne-Laure CassoneAnne-Laure Cassone work consists in performing flow-cytometry analysis to assess the impact of MP on oyster and particularly on their hemocytes. She is also involved in analyses of interactions between MP and Vibrio (Vibrio splendidus and V. aesturianus).

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ILVO  Ifremer  Centre national de la recherche scientifique (CNRS)  Cefas  Deltares, Enabling Delta Life  Provincie West-Vlaanderen, door mensen gedreven  Ministerie  Interreg IV A, 'Investing in your future' Crossborder cooperation programme 2007-2013 Part-financed by the European Union (European Regional Development Fund)